Bi et al 1999

Bi et al 1999 - Plant Cell Reports (1999) 18: 835840...

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Abstract Scented geranium ( Pelargonium sp. ‘Fren- sham’) was transformed with a gene encoding an antimi- crobial protein ( Ace -AMP1) from onion through an Agro - bacterium -mediated transformation system. The binary vector pFAJ3033 contained the coding region of the Ace -AMP1 preproprotein-encoding cDNA. Transformants were verified by polymerase chain reaction and Southern blot analysis. Transgenic plants expressing high levels of Ace -AMP1 were identified by immunoblots and those plants were shown to have increased resistance to Botry - tis cinerea leaf infection. Key words Geranium · Genetic transformation · Ace -AMP1 · Botrytis cinerea · Disease resistance Abbreviations MS Murashige and Skoog (1962) me- dium · PCR polymerase chain reaction · BA N 6 -benzylad- enine · NAA α -naphthaleneacetic acid · NPT II neomycin phosphotransferase II · DIG digoxygenin · Ace-AMP1 Al - lium cepa antimicrobial protein Introduction Geranium ( Pelargonium sp.) is an important ornamental crop throughout the world, and its sales are growing stead- ily. While the geranium industry is expanding, there are still many factors which limit their production signifi- cantly, one of which is the plant’s susceptibility to a va- riety of pathogens during all stages of growth and devel- opment. Gray mold, or Botrytis blight, of geranium, caused by Botrytis cinerea Pers.:Fr., is one of the important destruc- tive diseases affecting geranium production (White 1993) in causing stem, leaf and flower blight. The disease symp- toms are characterized by gray, fuzzy sporulating lesions commonly observed under humid conditions. Although B . cinerea infects all above-ground tissues of geranium, senescing tissues are particularly susceptible. The primary inoculum for the disease are the conidia, which are carried in air currents to uninfected tissues, and the mycelium present in fallen petals (Sirjusingh et al. 1996). A wet, hu- mid greenhouse environment provides a very favourable condition for the rapid growth and prolific sporulation of B . cinerea (Hausbeck and Moorman 1996). Fungicides are commonly used to control gray mold. However, this is becoming less acceptable since it in- creases the potential for the build-up of resistance in B . ci - nerea to fungicides and also conflicts with the public concern for fungicide residues (Hausbeck and Moorman 1996). Biological control, on the other hand, has advan- tages over fungicides, but its efficacy varies depending on the timing and the environmental conditions. Moreover, Botrytis cinerea still could develop resistance to biologi- cal control agents (Li and Leifert 1994). Traditional breed- ing for resistant cultivars has not been very successful so far, mainly because of a lack of host resistance to B . ci - nerea (Jarvis 1977). Genetic engineering of plants to introduce new disease
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Bi et al 1999 - Plant Cell Reports (1999) 18: 835840...

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