lab04 - 4-1 SPRING 2010 NAME...

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Unformatted text preview: 4-1 SPRING 2010 NAME _______________________________ BTNY21SECTION (DAY/HOUR)______________________ LABORATORY #4 PHOTOSYNTHESIS Lab #4 Objectives:1) learn the inputs and outputs of photosynthesis 2) investigate how input changes affect the outputs 3) study the pigments involved in conducting photosynthesis 4) learn how the primary product of photosynthesis can be used by the plant You will be working in groups of three this week. List your group members: Photosynthesis is broken down into two processes. The first one (which gets things started) is called the light dependent reaction of Ps. The second one (which results in the actual production of glucose) is called the light independent reaction of Ps. Write the balanced equation for photosynthesis. During todays lab we are going to examine the components involved in photosynthesis. By modifying the environment that a plant is growing in, you will learn about the impact that these components have on photosynthesis. 4-2 A. LIGHT DEPENDENT REACTION OF PHOTOSYNTHESIS All of you should have learned long ago that plants require light in order to make food to survive. You may have even experienced trouble when growing plants in an inadequately lit room or home. Todays first experiment will provide a visual demonstration of the importance of light to the process of photosynthesis. Each group will work with variegated Coleus plants that have been grown under different conditions for the last two days. One set of plants has been left to grow under normal greenhouse conditions while a second set of plants were placed in a growth chamber without lights. Therefore, the difference between the two sets of plants is that for the last two days one set was grown in the light while the other set was left in the dark. You will make leaf clearings (removal of the chlorophyll) and then stain the cleared leaves with I2KI to determine the distribution of starch throughout the leaf. Your TA will indicate the stations on the side benches for doing leaf clearings of light and dark grown plants. 1. Obtain and draw a leaf from the light and dark grown plants in the space below: Leaf (plant in light) Leaf (plant in dark) (distribution of chlorophyll)(distribution of chlorophyll)2. Place your leaf in a beaker of hot water for about 30 seconds. 3. Using forceps, transfer the leaf from the hot water into a beaker of 95% ethanol in a water bath. Leave the leaf in the alcohol until all or most of the pigments have been leached out. 4. Place the leaf in a Petri dish and pour a little I2KI over it. Make sure to spread your leaf out flat. I2KI is a brown liquid that stains starch black. 5. Remove the stained leaf and place it on a brown paper towel. At the top of the next page redraw your leaves, but indicate the distribution of starch this time. 4-3 Leaf (plant in light) Leaf (plant in dark) (distribution of starch)(distribution of starch)a) Describe the relationship between the distribution of starch shown above and the distribution of the chlorophyll that you drew on the previous page. distribution of the chlorophyll that you drew on the previous page....
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This note was uploaded on 02/23/2012 for the course BTNY 210 taught by Professor Staff during the Spring '08 term at Purdue University-West Lafayette.

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lab04 - 4-1 SPRING 2010 NAME...

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