lab09 - 9-1 SPRING 2010 BTNY 210 NAME _ LAB SECTION_...

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9-1 SPRING 2010 NAME _______________________ BTNY 210 LAB SECTION_________________ LABORATORY # 9 (MEIOSIS AND INHERITANCE IN PLANTS) Lab #9 Objectives: 1) learn the differences between how and where the processes of mitosis and meiosis occur 2) study basic flower structure and know which gametes are produced within which floral structures 3) become familiar with the following genetic terms: monohybrid cross, dihybrid cross, homozygous, heterozygous, dominant, recessive, genotype and phenotype 4) know how to perform monohybrid and dihybrid crosses from the parent generation to the F 2 generation and what the expected genotypic and phenotypic ratios are for each cross A. MICROBIAL GROWTH Before getting started with this week’s exercise, you are going to inoculate a couple of nutrient agar Petri plates for future use. Pickup two nutrient agar Petri plates to be inoculated. Your TA will demonstrate how to inoculate the plates. You may be as creative as you like. Use a cotton-tipped applicator to maybe swab your shoe, face or drinking fountain. Perhaps, place a hair or some saliva on the plate. Your other plate will serve as a control. If you used a swab from your shoe for the first plate, then swab part of the control plate with a fresh applicator. If you placed something on your plate, then leave your control blank. Label the plates with your name, how you inoculated them and place them on the tray on the side bench. We will store the plates and you will see what grows by lab time the week after Spring Break. B. MITOSIS vs. MEIOSIS Start by referring to page 236 in your textbook. Recall that mitosis begins with a single cell that divides into two genetically identical daughter cells. 1. Where do most mitotic divisions occur within a plant? 2. Does mitosis only take place at these locations? Meiosis is also a process of cell division; however, it is a very special kind of cell division. Meiosis starts with a single cell that divides to yield four genetically different daughter cells. These cells are genetically different in that each daughter cell only has half the amount of DNA as the parent, and the chromosomes themselves can have new combinations of DNA that is different from the parent. These daughter cells are called gametes .
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9-2 3. Name the two types of gametes and where they are formed within a flower? 4. What is the process that causes new combinations of DNA on the chromosomes? Use the figure on page 236 to see what happens when cells undergo mitosis and meiosis. In mitosis all chromosome line up on the equatorial plate and the chromatids are separated. Meiosis involves two divisions (Meiosis I and II).
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This note was uploaded on 02/23/2012 for the course BTNY 210 taught by Professor Staff during the Spring '08 term at Purdue.

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lab09 - 9-1 SPRING 2010 BTNY 210 NAME _ LAB SECTION_...

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