lab10 - 10-1 SPRING 2010 BTNY 210 NAME _ SECTION...

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10-1 SPRING 2010 NAME _______________________________ B T N Y 2 1 0 SECTION (DAY/HOUR)______________________ LABORATORY #10 (BACTERIA AND FUNGI) Lab #10 Objectives: 1) learn the major differences between prokaryotic and eukaryotic organisms 2) know and recognize the 3 bacterial shapes and become familiar with the interaction between legumes and nitrogen fixing bacteria 3) observe an example of a cyanobacterium and learn how to use a dichotomous key 4) study 3 major groups of fungi, learn some of the specialized terminology used with fungi as well as some of the characteristics that distinguish the groups from one another A. BACTERIA 1. HETEROTROPHIC BACTERIA: Heterotrophic bacteria cannot produce their own food. They feed on organic materials produced by other organisms in the environment. a) Are heterotrophic bacteria prokaryotic or eukaryotic? b) What are heterotrophic bacteria that decompose dead organisms called? STATION 1. BACTERIAL SHAPES c) List the three common shapes of bacteria: You will see tubes marked 1 and 2. Each tube contains one of the following species of bacteria: Staphylococcus or Rhodospirillum . It is your job to figure out which tube contains which bacteria. Examine the prepared slides and determine which species is in each tube. Sample #1 has been stained with Toluidine Blue to aid visibility. Examine under 40X magnification. Please make sure each person in your group looks at both slides. d) Based on your observations, what does each tube contain? 1 ) 2 )
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10-2 STATION 2. NITROGEN FIXATION Many plants in the bean family form symbiotic associations with bacteria that are able to use nitrogen from the atmosphere. This association begins when bacteria infect the plant root causing the plant to produce specialized structures called root nodules that “house” the bacteria as they grow and divide. Plants cannot use gaseous nitrogen directly, but they can assimilate reduced nitrogen made available by the bacteria. In return the plant provides nutrients and a stable environment for the bacteria. Bacterial nitrogen fixation in root nodules is the basis for crop rotation practices alternating corn and soybean to increase soil nitrogen. After soybeans are harvested, the root system breaks down and releases incorporated nitrogen into the soil. a) How does the plant benefit from infection by the bacteria? b) What do the bacteria get from the plant? c) What is the specific name for a symbiosis in which both partners benefit? d) Examine the root system of the soybean plant and locate the root nodules. Where are most of the root nodules located, scattered on the lateral roots or clustered along the taproot? e) Name two nitrogen-containing molecules that are essential to plants. f) Take a look at the electron micrograph showing a single cell within the nodule.
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This note was uploaded on 02/23/2012 for the course BTNY 210 taught by Professor Staff during the Spring '08 term at Purdue University-West Lafayette.

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lab10 - 10-1 SPRING 2010 BTNY 210 NAME _ SECTION...

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