Luminol - LUMINOL Source: "Organic Experiments"...

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1 LUMINOL Source: "Organic Experiments" by K. L. Williamson, D. C. Heath, 1989, pp 472-474. Synthesis of Luminol Place 3 mL of water in a 10 mL beaker and heat. (This will be used later) In a 4 inch test tube combine 200 mg of 3-Nitrophthalic acid and 400 micro liters of an 8% aqueous hydrazine solution (use plastic syringe). Heat the tube with a heating block until the solid dissolves. Add 600 micro liters of triethylene glycol to the tube. Clamp the tube in the heater. Insert a boiling chip and a Pasteur pipette connected with a hose to the aspirator. The end of the pipette will be near the top of the tube, not in the liquid. Its purpose is to remove water vapor as it is boiled off. Monitor the reaction temperature with a thermometer in the heater. Boil the mixture vigorously. Note that this will begin at about 110 ° C. After about 3-4 minutes the temperature should rise to 215 ° C. Maintain the temperature of the mixture between 215 and 220 ° C for two minutes. Remove the tube and cool to about 100
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Luminol - LUMINOL Source: "Organic Experiments"...

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