exam22011-key

exam22011-key - Payne, Biol 330 Exam #2 key 3/16/11 Print...

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Payne, Biol 330 Exam #2 - key 3/16/11 Print Your Name: Your Laboratory Section:   DAY     TIME Directions : 1. Print Your Name and Section on every page of this exam  NOW . 2. Use a  PEN  to complete this exam. If you use pencil, you may not be eligible for a  regrade. 3. Check to see that you have  6 sides on 3 pages . If you don t, raise your hand. 4. You  must  confine your answers to the spaces provided.  5.  Read  the questions! - Be as specific and as concise as you can. Use the  minimal  number of words necessary to completely answer the question. Use diagrams and  graphs if needed, but remember to label them! 6. You must hand in your exam by 9:50 AM. Good Luck. Scoring : Possible Pts. PAGE 2 /  20 PAGE 3 /  16 PAGE 4 /  16 PAGE 5 /  26 PAGE 6 /  22 TOTAL      /  100 I pledge on my honor that I have not given or received any  unauthorized assistance on this examination  Signed 1
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Name ______________________ Lab Section day ______________ time_____________ 1) Enzymes are catalysts: Name THREE general properties that they have in common with biological catalysts (6pts) Some possibilities: Are only required in small amounts must be left unchanged at the end of a reaction, so that it can cycle back to bind more substrate Catalyzes equally the forward and reverse reactions 2) Circle all of the techniques below that would enable you to determine that a protein, X,. was present in the mitochondria of liver cells. Engineered expression of GFP-tagged protein X in liver cells Immunocytochemistry (antibody labeling) of protein X in slices of liver cells Are correct – the others localize the protein to the tissue or cell, but not to mitochondria 3) A snake venom contains a powerful protease that attacks a critical enzyme in the cells of the prey and breaks up the enzyme into small peptides. You are investigating the in-vitro activity of the enzyme that gets attacked. Draw a graph showing the relationship between substrate concentration and the initial rate of the
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exam22011-key - Payne, Biol 330 Exam #2 key 3/16/11 Print...

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