Lab01-Pipettes-and-microscopes

Lab01-Pipettes-and-microscopes - BSCI 330 Laboratory Manual...

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BSCI 330 Laboratory Manual Spring 2012 Carpenter et al. 2011. BSCI330 Laboratory Manual. University of Maryland, College Park. Page 1 EXERCISE 1: PIPETTES AND MICROSCOPY Among your first tasks when writing in your lab notebook will be to record predictions you pose about the experiments you will be doing in lab. Recall that a prediction is a statement of expected results, based on some prior knowledge, observation or on a tentative explanation of a natural phenomenon ( hypothesis ). An example of a prediction might be, We predict that we will observe more protein in sample A than in sample B .” A prediction is not a question ( e.g. , “ Which sample will have more protein?” ), nor is it a statement of what you hope to study ( e.g. , “We would like to see if there is more protein under different conditions.”). The exercises in this lab period are not “true” experiments, but rather serve to offer technical practice, so you will be skilled at these techniques when you use them in experiments later in the semester. There are, however, a few instances in which you should be thinking ahead to the results you may see and thus formulating predictions. Those instances are emphasized within the text of this lab manual, as it is assumed that you will read the lab manual and complete the necessary parts of your lab notebook PRIOR to coming to lab. Review of Laboratory Technique From your previous experience in other biology courses, you should have been introduced to several important laboratory techniques. Although they should therefore already be familiar to you, we will review them briefly, as optimal success in many of the laboratory exercises this semester will depend upon their proper implementation. 1. Using the automatic pipette: Automatic pipettes come in several sizes: Size Maximum Capacity P-10 10 l P-20 20 l P-100 100 l P-200 200 l P-1000 1000 l P-5000 5000 l (*Recall 1000 l = 1ml) Figure 1.1 Diagram of a typical micropipette http://www.di.uq.edu.au/sparq/images/micropipette.jpg Nearly all biology research labs use automatic micropipettes; however, the utility of these instruments is only as good as one’s understanding of their proper use and manipulation. In addition, each automatic pipette costs several hundred dollars to purchase and further money to routinely calibrate. They must be treated gently to be of value in the lab. Therefore, this exercise will teach you how to
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BSCI 330 Laboratory Manual Spring 2012 Carpenter et al. 2011. BSCI330 Laboratory Manual. University of Maryland, College Park. Page 2 properly use and care for the pipettes in the lab. Remember that the success of your lab depends upon the proper use of the lab equipment! The Gilson Pipetman offers two-step pushbutton operation with simple one-handed pipetting.
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This note was uploaded on 02/27/2012 for the course BSCI 330 taught by Professor Payne during the Spring '08 term at Maryland.

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Lab01-Pipettes-and-microscopes - BSCI 330 Laboratory Manual...

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