Final Exam key 2011

Final Exam key 2011 - Ker [05‘ _ \ Payne, BSCI 330 Exam...

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Unformatted text preview: Ker [05‘ _ \ Payne, BSCI 330 Exam #4 gw a l 0.9 5/16/2011 , Print Your Name: Your Laboratory Section: DAY TIME Directions : 1. Print Your Name and Section on every page of this exam NOW. 2. Use a PEN to complete this exam or you will not be eligible for a regrade. 3. Check to see that you have 5 pages and 9 sides. If you don’t, raise your hand. 4. You must confine your answers to the spaces provided. 5. Read the questions! ~ Be as specific'and as concise as you can. Use the minimal number of words necessary to completely answer the question. Use diagrams and graphs if needed, but remember to label them! 6. You must hand in your exam by 9:50 AM. Good Luck. " . Scoring: Possible Pts. PAGE 2 / 28 PAGE 3 / 26 /,,,,2,6,, ,,W, a a" e / 20 / 34 / I i! / g“ r: / 18": a TOTAL: / 200 1‘ n 1 I. 'c Name SectioniiDay Time 1) Choose, from the list below (a—e), one appropriate microscopy method best used to solve each of the problems listed beneath the methods (f—j). USE EACH METHOD ONCE ONLY IN YOUR ANSWERS (i.e. linking method (a) to BOTH (t) and( j) is not allowed (10 pts) a) Bright field (i.e. conventional) light microscopy b) Freeze-fracture electron microscopy 0) Phase contrast microscopy d) A GFP—tag and fluorescence microscopy e) antibody labeling and fluorescence microscopy f) To show the position of a stained nucleus in a living, craning amoeba. g) To show the outline of unstained organelle membranes during cytoplasmic streaming h) To show that a protein is localized within the nucleus of a living cell i) To show the doughnut-shaped strctuure of the nuclear pore complex j) To show that a protein is localized to the Golgi apparatus in fixed slices of a cell Ans<e.g.a,c AA: ‘o,_i_ 2) Which THREE statements below are true of all cells? ( CIRCLE THE SQUARES TO THE LEFT OF THE TRUE STATEMENTS; 6pts, two pts off for each incorrect answer) : All cells have mitochondria . " p I) All cells store their hereditary information in DNA . All cells use proteins as catalysts 7 All cells have intermediate filaments Allcellstranslate’RNAinto*proteinetheesamewa n 7 r , min, , ,, ,7 7, 7 7 7 r if 7 " All cells have endoplasmic reticulum has a large, positive AGO, will not have equal quantities of product and reactant 3) Describe why a reaction that present when the reaction reaches equilibrium. Refer to specific equations and DEFINE ANY SYMBOLS AND CONSTANTS YOU USE. (Spts) be" cam a Km a, sea in). «mirtVfi ea 5 q“ 63‘) ‘1; W o g , can“ an. .a 3 "(g 1.56%. We.) . («By CPU New“ \Zzguum‘f; Ye o¥s¥¢u§;c%} th’hufi not. ; Cfixa TQMMV c A The maintenance of the ion gradients needed to create a cells’ resting membrane potential requires the constant activity of the Na+/K+ pump. Is this an equilibrium or a steady state? —~ EXPLAIN YOUR 0;“ mama. am (Mme gamma; WV? > ; é»%§$f.,,€§fe i Name‘ wwm Section: Day Time 5) A cytosolic protein becomes phosphorylated by a kinase. Answer the following questions. A) Do you think that the phosphorylated protein MORE OR LESS likely to translocate into the hydrophobic, core of the plasma membrane? EXPLAIN YOUR REASONTNG (4pts) I €:v\sc\%gi K} ’{o tyén QMWcQg‘fsék ‘ "i 'f'="“C “ti-6“: lfiaaafishfw ‘- wk mmmwuw: ; ‘ V ._ V fir}. w . ' B) What method would you sueth definitiyely deteine the . tertiary structure ofthe phosphorylatdd protein? Ans. - v .V (213$) " ' . a . C) In the presence of the kinase, 30% of the protein molecules are phosphorylated. Which state therefore has the lowest free energy ...... .. ' ' —Phosphorylated onphosphorylated? (CIRCLE ONE) (2pts) 6) Which ofthe following are TRUE of enzymically-catalyzed Swans? ( CIRCLE THE SQUARES TO THE LEFT OF THE TRUE STATEMENTS; 6pts, two pts off for each incorrect answer) I Adding enzyme to a reaction at‘equ‘ilibrium will result in more product formation Enzyme inhibitors always {bindrto the enzymeis actiige site V " Competitive inhibitors alter the affinity of an enzyme for its substrate Enzymic reactions can be sensitiye to pH ‘ . l Enzymic reaction are reversible because they utilize only weak bonding to the substrate Q Enzymes can become charged during the catalytic steps 7) In their experiment to calculate the number of monolayers in red blood cell why did did Gorter and Grendel have to know the NUMBER of red blood cells from which they extracted lipids? (4pts) 8) A) AG for net flux of calcium ions out of a cell is invariably large and positive — why is this? (4pts) ‘ D Claw}; fig {Co-“3a ' a) we \J “NC 6 fit}: at cams»... ‘6 CW?) «he. 2m «cng 7 B) Active transport of calcium out of animal cells is Usually much reduced when's’you replace extracellular sodium ions with another monovalent positive ion, like lithium — why do you think-this is (4pts)? ‘53” ‘ 3m?! at . «v "M L: ; \ Name __ Section: Day Time 9) A spider venom attacks the structure of voltage-gated sodium channels in a neuron and slows down the rate at which they inactivate. Sketch the time—course (“shape”) of an action potential before and after treatment of a neuron With this venom and explain Why the venom has the effect that you propose. (8pts). W .. 10) Which membrane has the greatest area — the inner membrane of chloroplasts or the thylakoid membrane? Briefly explain the reason for your choice (4pts). <k$\aws‘o " \‘9 “km” were . 9L 36 w“. Nos“ VUWQN 11) Answer the following questions about targeted nuclear export of proteins (8pts) a) Which nucleotide provides the energy for nuclear export? 7 b) Which three proteins make up the protein complex that travels through the nuclear pore complex ,. ‘ . during theme?“ ort process9 , s, . «who: 13;» may}? (fifip . 12) Amutation m a protein, eliminates the first ten ammo is at 1ts"N-tegmnal;"ln mutant mice expressing this mutant protein, the protein accumulates inth‘e‘ cytdsdl normal it“i‘spackaged into iv‘esiclesandé secreted from the cell. Based ONLY on your knovvledge of protein synthesis and vesicle transport pathgvays, as describedyinpflectureipresent a plausibleflhyp‘othesisthat explains the effect pf thetimutatioyn as Name) _ Section: Day Time 13) Describe ONE example of receptor-mediated endocytosis — describe the proteins and structures involved and their location in a cell (8pts) Ein \N W”¢§\Q£u 4 backmm'fiuk 4)'Whichispgces Within mitochondria and chloroplasts might you expect to béoorneWei'ygcharged due to it“ proton pumping (4 pts)? Chloroplasts L?!» o Mitochondria W&¥(\ h "39195) Describe h0Wfiight causes the niernbrane potential" of a god photoreceptor to hypgrpolarizeig Describe (in ' ’WO'rd’s’or' a’la'be'ied diagram)"the’ Broteins;’m§s’sen§érs"éiild'fi'onsl'iriiféolved,'Whereithefif are’ldcatedianckhgwthey' * i we interact to generate; the response (Spts); r r. . ' i " ' e . 5 3””- Namé ____ _______ Section: Day Time 16) Name THREE different examples of a type of cellular motility (e. g. swimming using cilia) or intracellular movement that were covered in our lectures that are dependent on the cytoskeleton. For each kind of motility, name the family of motor proteins‘used. You need not definejsubfamilies such as myosin I, II Illnetel‘Q pts for each correct PAIR)! ' 5' " ; ~ p: r 1V .. ,. fie , a. Motility: mménj w H‘W vfl Motor protein: D (hi/1t - TrawaaMr V ., \ . ‘n 0" b. Motility: \lcs‘wV’ H) k Meter; protein: w N” ‘ ’ “s M \ * 0M 0. Motili : ' *0 ° Motor rotein; r 7 W C ’Qr'gifi’gfiiagfie ' ' Yoga-{£4 17) Where would you find gamma (7) tubulins and yrhat is their function? (fillets). : v. i?“ :75! 2;- ‘l- a , 18) What stabilizes the (+) end of microtubules as they grow in a test tube? (4pts). ‘} t“ a» m “Wan m \ehfl K W W Gave ‘@ (at Sam 9 a 19) Name TWO structures that stabilize the (-) end of microtubules in cells (4pts)? « 20) What does the druggiéaxol do and why is it an antii‘Cancleriidrugg? (6pts). 93f" ’ éutlfla i of is keratin, whereas it broduced and what is its function? (opts) \ NVQJ ,4 ( WE») skim cask; i c 29“) S’fflvwsk‘wtmk a He». euém \a & W A m e a , Name ‘ « ‘SeCtioh: Day Time 23) Describe how the rueaseb‘f calcium ions the sarcbplasmic reticulum activates muscle contraction (8pts) Faulty 1M“ 25) Describe, in a labeled diagram, preferably, the structure of a typical cilium, shown in cross section. Label the proteins and structures and identify the motor proteins that drive movement (lOpts) I . 7 .yr‘ 7 ' /.E .1ng In Jule: w SMA-al much. :62} l 26) Name TWO cell types that do not divide once they mature ‘ I ‘U“’ (4pts) ‘ 27)]3‘3301‘ibe an experiment that proves that ajcytcplasmid'factor can trigger the tiansitidp of a cell from G1 to S phase (8pts). ‘ ' " - s a .1 » r V 3 t Name" __ Section: Day Time 24 *r _¢*"W \e‘ééwg w? fl 28)il)escribe classes 7 dependent kinases (4pts). . , . ' x 5 \amm‘f’ um swam, \W N yr}; h ‘% m‘ ‘95. m. , 0“ cw! 2) Rah 9. ‘ 29) What class of enzyme is a caspase? (as defined by the general class of reactions that it catalyses) 32) Describe the function (not the stmcture!) of a gap (or channel-forming) junction (4pts). (5 Mb» Shall MAMA” hi kwfi ‘5“va gihmé «é are te“ at. Mask Section: Day Time Namegw ____ _____l______ 33) Describe the mechanism by which a killer lymphocyte initiates the apoptosis of target cells m describe the proteins involved, their location and the sequence of events (lOpts). wm \eérgm 34) Describe TWO examples of events during embryonic development that show that apoptosis must occur (8 pts) ' 1. “Q afi'g We} ficfifi“ 7% x“ % >> .. wwm. ammmwgw «v33 .3 ...‘_mmwwwm$l$mw wmmmmugw awmmmmfim figmme firmnanfiommm a vjmmonfioflm mm m Seaman Ufionmmm. mmnmufiow Uwofimmsm m3 mnq<mfimg firm” H833: mfismfi 3 Em mm: Efimlow m3 Eamfim Em fimmvosmm. 3m Ummw-n:m$nflmlmmq gamma mwm mmmgmmmm mfimnjmg .8 55%3 82m $1853 _. ,. mm” 8:8 firm a 8% mm 883%va ".93; a $3 gm $5 .8: Swan 9n mmnyfmsucwo‘h $38 omAHEmQermgammymmmmgu:mm» Ercmm was magnmmgm 8: .mommfia QM _. Wmmm m% ,3. 333 m .mvymmmwggm gm ‘ P Ecco .qccécv. rcmznc Q =¢C Etc >c££m EEcEcE 5x-§ a: E mag gfiufifi wwm ammaw mmwfi. . mmfififiwfi mmmm .. «39$ mum . mmwww mfifiwmww mmmmw mmmmmgw mofimm ET! Emmng wfifimfig 3w 9% $an army. m mg wwmmwmmw mi.“ H.” m mfimfiwfimn wmwwmmw nmm 3 50. Axum ilbnibw 33353 2n :90. 3D: 39 32.331 .DDNDDDD v32», ...
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This note was uploaded on 02/27/2012 for the course MATH 2 taught by Professor Staff during the Spring '08 term at Maryland.

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Final Exam key 2011 - Ker [05‘ _ \ Payne, BSCI 330 Exam...

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