06 Enzyme Inhibitors

06 Enzyme Inhibitors - Enzyme Inhibitors Inhibitors alter...

Info iconThis preview shows pages 1–3. Sign up to view the full content.

View Full Document Right Arrow Icon
Enzyme Inhibitors Inhibitors alter enzyme activity by influencing the affinity for substrate (Km) or turnover number (Kcat). Inhibitors bind reversibly. Inhibitors are important physiologically, in controlling enzymes in the cell. In research – binding inhibitors may be used to investigate the active site. (see later lecture on mechanism of lysozyme) In medicine – many drugs are enzyme inhibitors. eg. Penicillin inhibits a stage in bacterial cell wall synthesis, preventing cell wall formation, and killing the bacteria.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Methotrexate – an enzyme inhibitor used as a drug. The chemotherapy drug methotrexate has a similar structure to the co-factor dihydrofolate. It binds to the enzyme dihydrofolate reductase inhibiting conversion of dihydrofolate to tetrahydrofolate. Methotrexate binds to dihydrofolate reductase 1000x more tightly than its natural substrate, making it a competitive inhibitor, but an extremely potent one. Tetrahydrofolate is essential for production of the DNA
Background image of page 2
Image of page 3
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 12

06 Enzyme Inhibitors - Enzyme Inhibitors Inhibitors alter...

This preview shows document pages 1 - 3. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online