Presentation11 - DNAStructure

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The genetic material has to be capable of transmission from one generation to the next with  high fidelity and yet be capable of variation. DNA is an un-branched, anti-parallel, negatively charged, double helix composed of four  bases-A, C, G, T.  The two strands are complementary.  A pairs with T and C pairs with G. A C G T A A C A G G T T G C A T T G T C C A bp= base pair kb= 1000 bp Mb= 1,000,000 bp Gb= 1000 Mb Sugar- phosphate  backbone H-bonds DNA Structure
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A C G T A A C A G G T T G C A T T G T C C A DNA Structure A C G T A A C A G G T T G C A T T G T C C A Denaturation Hybridization Salt Heat Salt Heat Hybridization= reannealing
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label Colony  identification “Southern” blotting DNA probe hybridization Chromosome  painting Repetitive  (telomere) sequence Unique  sequence
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Restriction Endonucleases EcoRI cuts the 6-bp palindrome GAATTC
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This note was uploaded on 02/26/2012 for the course AGRY 320 taught by Professor Stewert during the Spring '09 term at Purdue University.

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Presentation11 - DNAStructure

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