Presentation101 - (anddevelopmental)pathways ;Nobelprize Neurospora Product Enzyme HShomoserine Gene W X w x CYSTcystathionine Y y

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Genetics and biochemical (and developmental) pathways One gene one enzyme- Beadle and Tatum 1941; Nobel prize. Neurospora Product Enzyme Gene HS  homoserine W w OAHS  O-acetyl homoserine X x CYST  cystathionine Y HCYST  homocysteine Z MET  methionine If a loss-of-function mutation occurs in gene w, will the fungus grow on media lacking  methionine?
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1 2 3 4 5 6 7 8 1 2 4 6 Replica plating Complete media Minimal media +  amino acids Selective media and auxotrophic mutants, Neurospora
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1 2 3 4 5 6 7 8 1 2 4 6 3 5 Replica plating Complete media Minimal media +  amino acids Minimal media +  vitamins Selective media and auxotrophic mutants, Neurospora
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4 1 2 3 4 5 6 7 8 1 2 4 6 3 5 Replica plate Complete media Minimal media +  amino acids Minimal media +  vitamins Minimal media +  methionine Selective media and auxotrophic mutants, Neurospora
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1 2 3 4 5 6 7 8 Complete  media Min. + MET
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This note was uploaded on 02/26/2012 for the course AGRY 320 taught by Professor Stewert during the Spring '09 term at Purdue University-West Lafayette.

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Presentation101 - (anddevelopmental)pathways ;Nobelprize Neurospora Product Enzyme HShomoserine Gene W X w x CYSTcystathionine Y y

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