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Test II review - Characteristics of Watson-Crick model of...

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Test II (Genetics) Griffith, Avery and Messelson-Stahl experiments DNA packaging (organization) steps Nucleosome structure and composition Enzymes involved in replication and their function Diagram to show replication fork, different components, leading and lagging strand direction Components of plasmid and importance of selectable markers Termination of transcription; different scenarios when terminator factor or sequence is mutated Role of different IF (initiation factor) and EF (elongation factor) How to design probes to screen a library for unknown gene Diagram of cis-acting elements in pro- and eukaryotes Nucleotide structure
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Unformatted text preview: Characteristics of Watson-Crick model of DNA Alternative forms of DNA Types of sedimentation centrifugation Denaturation of nucleic acids, Tm, effect of UV light Factors affecting reassociation kinetics Different bacterial DNA polymerase and their properties Eukaryotic DNA polymerase Polytene and lampbrush chromosome Repetitive DNA sequences Different types of vectors in recombinant technology Steps of PCR Steps of Southern blot Sanger’s method of DNA sequencing Degeneracy and Wobble hypothesis Transcription in pro- and eukaryotes Translation in prokaryotes Post-translational modifications...
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