Unformatted text preview: markowitz (am45362) – 03b POST lab – huq – (47985) 1 This print-out should have 5 questions. Multiple-choice questions may continue on the next column or page – find all choices before answering. 001 2.0 points Your microscope has the resolving power to see the components of a cell but you want to distinguish these components to watch a liv- ing process occur. The best choice to achieve this would be: 1. Fix the cells and add a dye to stain cellular components in situ 2. Use antibodies with fluorophores at- tached 3. Any of these answers 4. Use phase contrast microscopy COR- RECT 5. Permeablize and stain the cells to im- prove resolution and contrast Explanation: 002 2.0 points The purpose of the dichroic (or mir- ror) in the fluorescence microscope is to w avelengthsoflightthatareemittedbythefluorophore. 1. absorb and reflect 2. transmit CORRECT 3. absorb 4. absorb and transmit 5. reflect Explanation: 003 2.0 points What is the purpose of immersion oil in brightfield microscopy?brightfield microscopy?...
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- Fall '07
- molecular biology, refractive index, resolution limit, microscopy CORRECT