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Unformatted text preview: markowitz (am45362) – 05b POST lab – huq – (47985) 1 This print-out should have 5 questions. Multiple-choice questions may continue on the next column or page – find all choices before answering. 001 2.0 points ¡p¿Which solution would make an ap- propriate spectrophotometric blank for the following enzymatic reaction?¡/p¿ ¡p¿Catecholnbsp;in Pi pH 7nbsp;buffernbsp;+ O¡sub¿2¡/sub¿nbsp;¡sup¿ < /sup><spanstyle =” text- decoration : underline ;” >tyrosinase</span><sup> < /sup>withCu<sup> 1. Pi pH 7 buffernbsp;+ melanin 2. water + benzoquione 3. Pi pH 7 buffer + Tyrosinase CORRECT 4. ¡span id=”choice r o- 28928” class = ” alignLeft ” style = ” display : inline ; ” > Catechol + Tyrosine < /span > 5. Catechol + Tyrosinase Explanation: ¡p¿N/A¡/p¿ 002 2.0 points ¡p¿During the chloroplast-isolation proto- col,nbsp;how were the chloroplasts initially released from the spinach leaves?¡/p¿ 1. The chloroplasted were released direct- lynbsp;from the leaves by differential centrifu- gation. 2. The leaves were mixed on a stir plate and magnetic stir bar with chloroplast reaction buffer....
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