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07b_POST_lab-solutions - markowitz(am45362 07b POST lab...

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markowitz (am45362) – 07b POST lab – huq – (47985) 1 This print-out should have 5 questions. Multiple-choice questions may continue on the next column or page – find all choices before answering. 001 2.0points ¡p¿Which one of the following levels of protein structure is LEAST likely to be disrupted or broken during the during a preparation of a crude protein extract? ¡/p¿ 1. hydrogen bonds of the secondary struc- ture 2. peptide bonds of the primary structure CORRECT 3. hydrophobic interactions of the quater- nary structure 4. the variety of bonding interactions of tertiary structure 5. All of these bonds are equally affected Explanation: ¡p¿bond¡/p¿ 002 2.0points ¡p¿What might be the outcome if PIPES was left out of the reaction mixture for preparing a crude protein extract? < br/><br/></p> 1. The proteins might become oxidized during the reaction procedure. 2. The proteins might have charged func- tional groups that are not normally present in their structure and cause denaturation. COR- RECT 3. The cellrsquo;s natural proteases might
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