08b_POST_lab-problems - 2.0 points pWhich oF the Following...

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markowitz (am45362) – 08b POST lab – huq – (47985) 1 This print-out should have 5 questions. Multiple-choice questions may continue on the next column or page – fnd all choices beFore answering. 001 2.0 points ¡p¿Native and denaturing gels have all oF the Following compnents in common, however, a native gel would NOT have. ..¡/p¿ 1. Chloride ions 2. Glycine bu±er 3. Hydroxide ions 4. beta-mercaptoethanol 5. Bromophenol blue dye 6. Resolving gel 7. Water 8. Glycerol 002 2.0 points ¡p¿Which one the Following is characteristic oF the stacking gel but NOT ¡em¿¡/em¿ char- acteristic oF the resolving gel?¡/p¿ 1. Higher concentration oF glycine 2. Lower percentage oF bis-acrylamide 3. Smaller pores 4. pH 8.8 5. Greater volume 003
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Unformatted text preview: 2.0 points pWhich oF the Following would allow proteins to migrate ASTER using SDS-PAGE?/p 1. Decrease concentration oF Tris-glycine 2. Increase percentage oF bis-acrylamide 3. Decrease current 4. Increases thickness oF gel 5. Decrease pore size 004 2.0 points pWhat reagent will be used to stain the liver and tripe proteins in the polyacrylamide gel?/p 1. BradFords reagent 2. Bromophenol blue 3. Any oF these answers 4. Methylene blue 5. Coomassie blue 005 2.0 points pWhich oF the Following would migrate to-ward the ANODE during gel semi-dry elec-troblotting?/p 1. Dodecyl sulFate ion oF SDS 2. Tris buer 3. Water 4. Glycerol 5. Glycine buer...
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This note was uploaded on 02/28/2012 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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