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Unformatted text preview: markowitz (am45362) 09b POST lab huq (47985) 1 This print-out should have 6 questions. Multiple-choice questions may continue on the next column or page find all choices before answering. 001 2.0 points pYou have discovered a new protein that is very similar to hemoglobin in structure and function.nbsp; First you run a native gel and isolate a single protein band.nbsp; You ex- tract the protein from the native gel and run the sample using SDS-PAGE.nbsp; You stain this denaturing gel with Coomassie and de- tect two distinct bands of different migration distances.nbsp; From these results you know your protein consists of:/p 1. one peptide that denatures and hy- drolyzed into two fragments seen as two dis- tinct bands. 2. one protein made of 4 subunits, however, only two survived the extraction procedure. 3. multiple peptides of two differently sized subunits seen as two distinct bands. 4. Nothing definitive can be said because two different gels were used. 5. two different-sized proteins, each made of multiple subunits. 002 2.0 points pA control Western blot with exposure to secondary antibody only (without any addi- tion of primary antibody) is compared to a typical experimental Western Blot in which...
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This note was uploaded on 02/28/2012 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.
- Fall '07
- molecular biology