11b_POST_lab-problems - markowitz (am45362) 11b POST lab...

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Unformatted text preview: markowitz (am45362) 11b POST lab huq (47985) 1 This print-out should have 5 questions. Multiple-choice questions may continue on the next column or page find all choices before answering. 001 2.0 points pWhat part of a transformation process was done on ice?/p 1. All of these answers 2. Incubation with calcium chloride to make cells chemically competent 3. Incubation of chemically competent cells with plasmid DNA 4. Initial mixing of chemically competent cells with plasmid DNA 5. Immediate treatment of the E. coli cells after heat-shock 002 2.0 points pRestriction enzymes are useful in creation of genetically engineered (recombinant) DNA for cloning because they can/p 1. recognize and hydrolyze the same spe- cific sequences of DNA in the same way re- gardless of the source. 2. All of these answers. 3. cut DNA regardless of source into the same size fragments with sticky-ends....
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This note was uploaded on 02/28/2012 for the course BIO 205L taught by Professor Hanson during the Fall '07 term at University of Texas at Austin.

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