Chem Proposal

Chem Proposal - Add potassium ferricyanide and a-hydroxy...

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By: John Frutiger Chemistry 103 - Lab Section  26A
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Key Words Lactic Acid Luminol Chemiluminescence Anaerobic Activity My aim is to accurately determine the concentrations of lactic acid in  solutions and be able to show that there is a relationship between the  intensity of their chemiluminoscity and concentration
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Pictures From: http://www.bmrb.wisc.edu/metabolomics/gen_metab_summary_5.php?molName=L http://www.3dchem.com/imagesofmolecules/lactic-acid.jpg
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Procedure (Briefly) Dissolve different amounts lactic acid in deionized water to  make different concentrated solutions
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Unformatted text preview: Add potassium ferricyanide and a-hydroxy acid oxidase to the solution Add luminol, which will react with the oxidized lactic acid (H2O2) to produce chemiluminescence Observe intensities of chemiluminoscity of each solution and compare to concentration Applications to Real Life Numerous applications already, mostly professional athletes get tested Usually tested by a pH test of blood. Direct correlation between pH and lactic acid levels Picture From: http://www.lactate.com/pitesadv.htm Picture From: http://www.mdconsult.com/das/patient/body/139582181-3/0/10041/35542.html THE END!!...
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This note was uploaded on 02/28/2012 for the course CHEM 103 taught by Professor O'halloran during the Fall '08 term at Northwestern.

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Chem Proposal - Add potassium ferricyanide and a-hydroxy...

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