DnaisoLAB - Name Layla Fijany Lorena Sahar Bio 3A AM DNA Isolation Worksheet Part A DNA Isolation 1 Record the exact weight of plant tissue you

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Name: Layla Fijany, Lorena, Sahar DNA Isolation Worksheet Bio 3A – AM Part A: DNA Isolation 1. Record the exact weight of plant tissue you started with: ______ 52.09 g ______ 2. Record your observations of the DNA extraction solution at the following steps: a. After the homogenate buffer and heating to 60 °C. green chunky with most of cloudy buffer settled on top b. As ethanol and aqueous layers are being “stirred” together. Light green foamy smoothie c. Appearance of DNA spooled onto glass rod. Thin stringy like, dissolved easily though, not that much 3. It is apparent that the plant cells contain a huge amount of DNA. How can so much DNA fit into the nucleus of a plant cell? Because of DNA efficient “packing” system. The DNA molecule is actually wrapped around histone proteins forming nucleosomes that are further coiled and looped to condense into chromatin, which make up a chromosome. It is this wrapping and condensing process that allows for DNA to be hundreds and thousands of feet long when unwrapped. 4.
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This note was uploaded on 02/28/2012 for the course BIO 3A taught by Professor Huntley during the Spring '10 term at Saddleback.

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DnaisoLAB - Name Layla Fijany Lorena Sahar Bio 3A AM DNA Isolation Worksheet Part A DNA Isolation 1 Record the exact weight of plant tissue you

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