dnaLAB - Biology 3A Laboratory DNA Isolation Worksheet...

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Biology 3A Laboratory Name: DNA Isolation Worksheet Part A: DNA Isolation 1. Record the exact weight of plant tissue you started with: ________________ 2. Record your observations of the DNA extraction solution at the following steps: a. After the homogenate buffer and heating to 60 °C. b. As ethanol and aqueous layers are being “stirred” together. c. Appearance of DNA spooled onto glass rod. 3. It is apparent that the plant cells contain a huge amount of DNA. How can so much DNA fit into the nucleus of a plant cell? 4. What was the function of the detergent in the homogenation buffer? 5. Which of the plant materials yielded the best results? Why do you think that plant material worked the best? 6. Briefly describe the appearance of the spooled DNA for the various plant materials. Biology 3A Lab DNA Isolation Page 1 of 3
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7. Does the quantity of the DNA differ between the onion, the peas, banana and carrot? Do you think the actual structure of the DNA molecules differs among the three test organisms? 8.
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dnaLAB - Biology 3A Laboratory DNA Isolation Worksheet...

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