Unformatted text preview: placed in a 10 mL-graduated cylinder and was filled up to 10mL with DI water. Percent solutions and agar (liguid) were placed in the autoclave to be sterilized. Forty Petri dishes were filled with agar and once hardened, 0.3 mL of Staphylococcus aureus was transferred onto each plate using aseptic technique. Three chads were placed on each agar plate with 5 µL of corresponding solution, that were placed equidistance from each other in a traingle formation. After all the agar plates were completely prepared, they were placed in an incubator set at 37 ˚C. Trial 1 was incubated for 45 hours; trial 2 and 3 were incubated for 18 hours. After the incubation period, zones of inhibition were measured (or lack thereof). Results There were no zones of inhibition present; therefore the mean diameter of the growth inhibition was 0.0 cm. This result was observed for all three trials, included 5%, 10%, 20%, 30%, 40%, 50%, 80%, 90%, 100% and for all of the water control experiments as well....
View Full Document
- Spring '10
- Biology, Agar plate, Petri dish, DI Water, Agar, Layla Fijany, Sahar Meshkat