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RespirationLAB

# RespirationLAB - Biology 3A Laboratory Cellular Respiration...

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Biology 3A Laboratory Name: Layla, Alexa, Garett Cellular Respiration Worksheet Lab Section: Bio 3A – Morning Lab A. Aerobic respiration in germinating peas 1. Upload the data from the Pasco Xplorer LX to Excel and graph the CO 2 production data as a function of time for the normal and the freeze/thawed peas. Add a trendline with the slope of the line equation and the R 2 value for both types of peas. Calculate the slope of each line and enter them on Table 1. The slope of the line is the respiration rate (ml/min or ml•min -1 ). Calculate the mass-specific CO 2 production. Table 1. Mass specific carbon dioxide production (ml/min/g) for normal and freeze/thawed peas Tube Contents Slope (ml/min) Peas mass (g) Mass-specific CO 2 production (ml/min/g or ml min -1 g -1 ) 1 Normal peas 6.28x10 -3 6.28 1.00x10 -3 2 Freeze/Thaw 3.50x10 -4 2.94 1.19x10 -4 Figure 1. Graph displaying CO 2 production versus time in normal peas. Trend line slope equal to respiration rate of 6.28x10 -3 ml•min -1 . Time and CO 2 production are directly related: as time increased, CO 2 production increased as well. Figure 2. Graph displaying CO 2 production versus time in frozen peas. Trend line slope equal to respiration rate of 3.50x10 -4 ml•min -1 . Time and CO 2 production are directly related: as time increased, CO 2 production increased as well.

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