LAB 6 - DNA Technology_student_2011

LAB 6 - DNA Technology_student_2011 - LAB 6 - DNA...

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LAB 6 - DNA Technology and Genetic Engineering
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Nucleic acids Nucleotides- made up of 3 subunits (SUGAR, PHOSPHATE AND NITROGENOUS BASE Each nucleotide takes on the name of the nitrogenous base Nucleic acid is a polymer of nucleotides DNA and RNA differ in the sugar backbone Nitrogenous
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DNA Structure D eoxyribo n ucleic A cid Double-stranded helix molecule Phosphate-sugar covalently linked form backbone The nitrogenous bases H-bond between the two strands Two anti-parallel strands 5’-3’ direction vs. 3’ - 5’ direction
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Complementary strands Two Purines (2ring) Adenine Guanine Two Pyrimidines (1ring) Thymine Cytosine Chargaff’s rule: 1:1 ratio of pyrimidine to purine %A = %T and %G = %C Watson-Crick double-helix model: complementary base-pairing A-T and G-C Chargaff’s rules
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Activity 2 DNA Extraction Cold Ethanol DNA isolation from plant (onion) cells 1. Cell lysis Break open your cells Blender- mechanically breaks open cells
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This note was uploaded on 02/28/2012 for the course BIO 220 taught by Professor Morre during the Spring '09 term at USC.

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LAB 6 - DNA Technology_student_2011 - LAB 6 - DNA...

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