Unformatted text preview: 2. Smear the bacteria on a slide and add a drop of hydrogen peroxide. 3. Observe the slide for quick bubble formation with mean catalase is produced. Procedure for Coagulase test 1. Put a drop of plasma on a slide then mix with a loop of Staph. Aureus. 2. Mix with inoculating loop and repeat procedure with Staph epidermidis Procedure for identification of staphylococcus: 1. Label plate with correct info and streak with Enterococcus faecalis 2. Label plate with correct info and streak with S. pyogenes 3. Sterilize forceps and add an A disc to second quadrant of S. pyrogenes streak plate. 4. Label plate with correct info and streak with S. pnuemoniae 5. Sterilize forceps and add a P disc to second quadrant of S. pnuemoniae streak plate. 6. Streak a slant with s. pyrogenes 7. Streak remaining slants with E. Faecalis 8. Put plates in correct location for incubation...
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- Spring '11
- Biology, correct info, Catalase, catalase negative streptococci, Label plate