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Microbiology 251-11 Labs 1-2

Microbiology 251-11 Labs 1-2 - Microbiology251...

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Microbiology 251 Laboratory Manual Spring 2011 Robert Twarog Denise Dalton Bruce Alexander
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Department of Microbiology and Immunology The University of North Carolina - Chapel Hill, NC MCRO 251 Laboratory Manual ii
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Preface Unlike most lab manuals published by textbook publishers, the Microbiology 251 Laboratory Manual describes  the laboratory activities exactly as they are to be carried out here at UNC-CH. The various species of  microorganisms and items of equipment described are those which will be used in the exercises. This should  make it easier to focus on the principles demonstrated in each activity which makes the lab manual a more  effective addition to the lecture. The experiments have been selected to introduce you to a variety of key  laboratory techniques and principles concerned with bacterial isolation and growth, impact of viruses on their  host cells, measuring immune response, etc. Once these basic techniques related to bacterial growth are  mastered, they will be applied to examine the effects of antibiotics/chemicals on bacteria. Along the way, you  will be given opportunities to apply these principles and procedures to the identification of unknown bacteria. Located on each of the benches in the lab room, A Photographic Atlas for the Microbiology Laboratory, by  LeBoffe and Pierce (Morton Publishing) may be used as a supplement. It features numerous photomicrographs  and extensive color plates of bacterial growth on various media. In addition, the underlying biochemical  principles of the laboratory tests used in the Microbiology 251 labs are discussed in detail. This combination of  a well-designed supplement with an in-house lab manual should be helpful. The laboratory exercises in this manual reflect the overview nature of the Microbiology 251 course. Though  most of the labs relate to the techniques and principles of bacteriology, there are also exercises devoted to  immunology and virology. Each section designated as a weekly lab session begins with the exercises to be  completed and evaluated from the previous week. The discussion then moves into the new material for the week  with a brief introduction for each exercise. Following the introduction, the specific materials used and  procedures   to   be   followed   are   described.   In   addition,   tables   to   record   data   and   questions   to   assess  understanding are also included where appropriate.  During   the   twelve   lab   sessions   and   the   unknown   identification   process,   you   should   acquire   a   basic 
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