Use, and the Standard Curve
Dilute a concentrated stock solution to a working concentration.
Accurately and precisely use pipettes in a laboratory setting
Use a spectrophotometer
Generate and evaluate a standard curve.
Use a standard curve to determine the concentration of protein in milk.
Closed-toe shoes must be worn
Long hair tied back
Goggles must be worn at all times while experiments are being conducted in the classroom.
if you’re done.
Gloves should be worn while performing the experiments.
All pipet tips should be ejected into the tip waste bin on your bench.
All Bradford reagent should be poured into the acid waste container on the side bench.
to put the lid back on when you are done.
Rinse your plastic cuvettes in the sink and place them in the trash can in the front of the room.
Lab Activity 1: Proper pipetting technique
Your Lab Instructor will demonstrate the proper technique for using a micropipettor.
When instructed following the demonstration, pick up a micropipettor (it does not matter what
size) and place the correct size tip on it.
Depress the plunger with your thumb and become accustomed to the "first stop" and the "second
Practice depressing the plunger to the first stop and s l o w l y releasing it back to the end stop.
When your Lab instructor comes to visit your table and instructs you to do so, pipet the volume of
water requested by your instructor from the container on your table into a microcentrifuge tube.
Under no circumstances should you ever dial a micropipettor above or below its stated volume
range! (Look at the knob—it will tell you the range!) Doing so will damage the pipettor and likely
make it extremely unreliable.
Your instructor may request that you repeat this process until you get it right. It's ok! It's practice
and you want to become proficient.