CHE1131Microscopy2

CHE1131Microscopy2 - CHE113 Forensic Science Nicolas Canel...

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CHE113 Forensic Science Nicolas Canel Microscopy Microscopy History •Dutch spectacle-makers (1590), Janssens, discovered that nearby objects appeared greatly enlarged with lenses. •Galileo (late 1600s), based on the Janssens experiments, worked out a much better instrument with a focusing device. -Leeuwenhoek Microscope -Hooke Microscope 1670 -Olympus (modern) -Pacino 1870 Compound Light Microscope The most common microscope - compound light microscope (LM). Two sets of lenses: ocular and objective. Brightfield Mic. The total magnification: multiply magnification of the objective lens with the ocular lens. e.g., ocular is 10x and the objectives is 100, total mag. will be 1000x. Optical system comprised of condenser, objective lens, eyepiece lens and illuminator. The compound light microscope uses visible light. ( λ = 400 - 700). Virtual image is any specimen viewed through a lens. Electromagnetic Radiation Magnetic and Electronic Parts mutually perpendicular Darkfield Microscopy The darkfield microscope shows a light silhouette of an organism against a dark
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CHE1131Microscopy2 - CHE113 Forensic Science Nicolas Canel...

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