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ch19 Rev. ATP synthesis RKW

ch19 Rev. ATP synthesis RKW - Lecture Connections 19 |...

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Lecture Connections 19 | Oxidative Phosphorylation and Photophosphorylation © 2009 W. H. Freeman and Company
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Proton-motive Force electrochemical proton gradient created by: actively transported protons across the membrane passing electrons to coenzyme Q that picks up protons from the matrix –electrons and protons from QH 2 are released to the inter-membrane side
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Chemiosmotic Model for ATP Synthesis Electron transport sets up a proton-motive force Energy of proton-motive force drives synthesis of ATP
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Free Energy in NADH
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Question Calculate the amount of energy conserved in the proton gradient across the inner mitochondrial membrane per pair of electrons transferred through the respiratory chain from NADH to oxygen. Assume Δψ = 0.15 V and the pH difference is 0.75 units
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Driving force for ATP synthesis Difference in proton concentration Difference in Charge
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Experiment Isolate mitochondria Give ADP, Pi, succinate What do you expect to see?
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