biochemistryexam3 - Membrane transport 1. Know the...

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Membrane transport 1. Know the mechanisms of facilitated diffusion. Glut1 transporter 2 mechanisms: CO2 producing system and bicarbonate fluoride anti- system -know how RBCs carry the components to the tissues of the lungs and exchange them in the bicarbonate chloride system -question on one system 2. Be able to make mechanistic comparisons of the active transport systems, i.e., be able to distinguish primary and secondary active transport, know the directionality of relevant ion movements and how they are energetically driven. -explain mechanisms -primary active transporters: ABC transporters (Cystic firbrosis transmembrane regulator which is normally a chloride channel), the Calcium ATPase -secondary active transport system: lac carrier protein, lac permease -energized by a cotransport with protons -know common mechanisms- involved in inactivation & active transport systems in which there is a conformational change which comes down to opening up the transporter of one bacteria in tert-plasmic space to take up the substance then the energy changes the conformation and decreases the affinity for that ligand inside and it is dumped out -first demonstrated with Calcium ATPase 3. Know the mechanism of H 2 O uptake by aquaporins. -know mechanism of how chains of water and the various charge distributions are able to exclude protons; otherwise the proton gradient buildup would spill out of the cell -size-dependent -also energization mechanisms -(briefly presented) mechanism of bacteria opsin which is a light transducent proton pump -know the direction of protons, the way they are moved out of the cell by bacteria opsin -first mechanism discussed in detail, arising from the structure of the protein -everything comes down to the mechanism being a proton wire -protons go down to the substrate channel in a sequence -discussed residues involved -all set off by an isomerization of the retinene -Cystic fibrosis regulator -one multiple choice question Photosynthesis* 1. Know the basic equations of photosynthesis and especially the Z-Scheme for the functional relationships between PSII and PSI; -know the sequence of electron carriers in the reaction centers of PSI and PSII 2. Know the sequence of electron flow in PSI and PSII; 3. Know the functional protein components and cofactors (and their sequences) of PSI and PSII;
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-cofactors of PI which are on the PSA A side with the exception of the first Chlorophyll which is on the PSA B side -two different PSA subunits -in PSII there is a DI and DII polypeptide to which these cofactors are attached 4. Know the overall arrangements and energy transfer pathways of the LHC-II and PSI and PSII light-harvesting complexes ; -know the sequence of the NAD transfer pathways from LHC-II and within PSI -know the pathways from LHCI to P700 Chlorophyll pair & the LHCII can move and transfer and then be phosphorylated, excitations occur and burn up PSII unless the excitations are not directed to PSII which is done by a phosphorylation mechanism -LH Chlorophylls (about 80) attached to PSA A and PSA B polypeptides
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This note was uploaded on 03/07/2012 for the course 830 201 taught by Professor Leyton during the Fall '08 term at Rutgers.

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biochemistryexam3 - Membrane transport 1. Know the...

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