enzyme lab report

enzyme lab report - Esther Kang November 9, 2010 Section D1...

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Esther Kang November 9, 2010 Section D1 Effects of the Enzyme Concentration of Alkaline Phosphatase (purified from chicken intestines, Gallus domesticus ), on the Reaction Rate of p-Nitro-Phenyl Phosphate (pNPP) I. Abstract : The enzymatic activity of alkaline phosphatase on the substrate, p-Nitrophenol, was assayed for each segment of the experiment. In order to do so, the absorbance was measured for the p-Nitrophenol with different diluted amounts the enzyme. To measure the effect of enzyme concentration on the chemical reaction, the rate of reaction would have to be found. The results showed that the solution with a concentration of 1 mL of the enzyme had a rate of .5 absorbance/10 mininutes, a solution with a concentration of .5 mL had a rate of .28 absorbance/10minutes, and a solution with a concentration of .25 mL had a rate of .14 absorbance/10 minutes. Additionally, the solution that had a concentration of .125 mL, .0625 mL, and .0375 mL of the enzyme had rates of .07 absorbance/10 minutes, .04 absorbance/10 minutes, and .01 absorbance/10 minutes, respectively. Based on the results of the experiment, the rate of the reaction increased as the enzyme concentration also increased; yet, the rate decreased when enzyme inhibitors were added. The results proved that the higher the enzyme concentration, the faster the rate of reaction; thus, a high enzyme concentration would result in a chemical reaction that would occur quicker than a solution with a low enzyme concentration. These findings show that the rate of
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enzymatic reactions is highly dependent on the concentration of the enzyme. II. Introduction : Most enzymes are proteins that catalyze reactions in various ways by lowering the energy of activation (E A ). Substrates bind to the enzyme at the active site, where they undergo chemical reactions, which transform them into products. The rate of enzymatically-catalyzed reactions is greatly reliant on factors such as enzyme and substrate concentration. Obviously, the rate of reaction for the reactions are not continuous; at some point, the rate of change in one direction is exactly the same as the rate of change in the opposite direction, thereby preventing the concentrations of the reactant and products from changing. This state is called dynamic equilibrium. At dynamic equilibrium, the difference in free energy between the reactants and products is zero (Solomon et al ., 2008). Shahid Ashrafi was a scientist who wanted to test whether the alkaline phosphatase activity in the locusts increased as its concentration also increased; and so, he performed an experiment in which he tested the alkaline phosphatase activity in the digestive system of a group of locusts
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This note was uploaded on 03/07/2012 for the course 830 201 taught by Professor Leyton during the Fall '08 term at Rutgers.

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enzyme lab report - Esther Kang November 9, 2010 Section D1...

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