13811 - Allomed Pharmaceuticals D-Chiro-Inositol and its...

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Unformatted text preview: Allomed Pharmaceuticals D-Chiro-Inositol and its Role in Diabetes and Insulin Resistance Joseph Larner The Search for an Insulin Mediator • Two Enzyme Bioassays • Initial Mediator Hypothesis and Search • Inositol Glycans Isolated from Rat Liver • Scale up to Beef Liver • Isolate and Determine Novel Structure of Pinitol Galactosamine Pseudodisaccharide Mn ++ Chelate o Bioactivity of Synthetic Pseudodisaccharide • Theoretical Docking and Allosteric Mechanism of Action of Pseudodisaccharide to Activate PP2C • Pathophysiology of Inositol Imbalance and Relation to Insulin Resistance • Epimerization Defect • Repletion with D-Chiro-Inositol • Future New Possible Therapeutic Agents: o Dibutyryl D-Chiro-Inositol o Pseudodisaccharide o Modified Pseudodisaccharide PDH (% Maximum Activity) TLC chromatogram of 2 purified inositol glycans from rat liver. TLC plate developed in isopropanol:pyridine:acetic acid:H 2 O (8:8:1:4) sprayed with ninhydrin. Lanes 1 and 4, galactosamine standards; Lane 5, PDH phosphatase stimulator from control rat; Lane 6, PDH phosphatase stimulator from insulin treated rat; Lane 2, cAMP-kinase inhibitor from control rat; Lane 3, cAMP-kinase inhibitor from insulin treated rat. TLC analysis of silica column...
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13811 - Allomed Pharmaceuticals D-Chiro-Inositol and its...

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