bioex_lect14

bioex_lect14 - This means that GFP-tags can be expressed in...

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MIT OpenCourseWare http://ocw.mit.edu 5.111 Principles of Chemical Science Fall 2008 For information about citing these materials or our Terms of Use, visit: http://ocw.mit.edu/terms .
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2008 Nobel Prize in Chemistry Discussed at the start of lecture #14 (not in notes or in the syllabus) The 2008 Nobel Prize in chemistry was awarded to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their work on Green Fluorescent Protein. The molecular structure of Green Fluorescent Protein (GFP) (GFP figures are from the Protein Data Base (PDB) accession number 1GFL) The chromophore in the center of the GFP protein. GFP is a 238-amino acid fluorescent protein that was first isolated from the jellyfish Aequorea Victoria. The chromophore of GFP (highlighted in magenta) is formed from natural amino acids.
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Unformatted text preview: This means that GFP-tags can be expressed in vivo, making them ideal fluorescent tags in biological systems. The GFP chromophore is NOT fluorescent on its own, so the entire GFP protein must be used as a fluorescent tag. This is because the chromophore is environment sensitive and the fluorescence is quenched in a polar environment, such as water. Protein engineering has resulted in the development of a rainbow of other GFP analogs with a range of excitation and emission wavelengths. For an interview with the 2008 Nobel Laureates see: http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/shimomura-interview.html Figure by MIT OpenCourseWare....
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This note was uploaded on 03/02/2012 for the course CHEM 5111 taught by Professor Vogel during the Fall '08 term at MIT.

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bioex_lect14 - This means that GFP-tags can be expressed in...

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