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Unformatted text preview: This means that GFP-tags can be expressed in vivo, making them ideal fluorescent tags in biological systems. The GFP chromophore is NOT fluorescent on its own, so the entire GFP protein must be used as a fluorescent tag. This is because the chromophore is environment sensitive and the fluorescence is quenched in a polar environment, such as water. Protein engineering has resulted in the development of a rainbow of other GFP analogs with a range of excitation and emission wavelengths. For an interview with the 2008 Nobel Laureates see: http://nobelprize.org/nobel_prizes/chemistry/laureates/2008/shimomura-interview.html Figure by MIT OpenCourseWare....
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This note was uploaded on 03/02/2012 for the course CHEM 5111 taught by Professor Vogel during the Fall '08 term at MIT.
- Fall '08