Lect20 - 1 Lectures 20 Amino Acid Biosynthesis 2 Important...

Info iconThis preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: 1 Lectures 20 Amino Acid Biosynthesis 2 Important concepts 1. Nitrogen fixation reducing inert nitrogen gas to 2 ammonia molecules Microorganisms 2. Memorize how the non-essential amino acids are synthesized Understand the aminotransferase mechanism (Schiff base; pyridoxal phosphate) 1-C unit carriers (THF, SAM) Nitrogen fixation reducing inert nitrogen gas to 2 ammonia molecules Microorganisms: For example: symbiotic Rhizobium bacteria in the roots of leguminous plants 3 Newly fixed nitrogen on earth: Nitrogen-fixing microorganisms: 10 11 kg N 2 /year, 60% Lightning and ultraviolet radiation: 15% Industrial processes: 25% Nitrogen fixation: nitrogenase complex •Nitrogenase inactivated by O 2 •Leghemoglobin binds to O 2 to keep free [O 2 ] low in the leguminous plants •Reduced ferredoxin comes from photosynthesis or oxidative processes •Reduction of molecular nitrogen N 2 to 2 ammonia (NH 3 ) N 2 + 8 e- + 8 H + + 16 ATP+16H 2 O -> 2NH 3 + H 2 + 16 ADP + 16 P i 4 Molybdenum-iron protein 5 Nitrogenase complex: reduction of N 2 Nitrogen fixation: ammonium ion assimilated into amino acids through glutamate and glutamine Schiff base •Catalyzed by glutamate dehydrogenase •Either NADPH or NADH 6 1st ammonium Schiff base Formed in many stages of amino acid biosynthesis and degradation 7 Nitrogen fixation: glutamine •Catalyzed by glutamine synthetase •Driven by ATP hydrolysis •Glutamine is an important nitrogen donor 8 2nd ammonia 9 Important concepts 1. Nitrogen fixation reducing inert nitrogen gas to 2 ammonia molecules Microorganisms 2. Memorize how the non-essential amino acids are synthesized Understand the aminotransferase mechanism (Schiff base; pyridoxal phosphate) 1-C unit carriers (THF, SAM) 10 Amino acids synthesis...
View Full Document

This note was uploaded on 03/04/2012 for the course CHEM 114B taught by Professor Wang during the Spring '09 term at UCSD.

Page1 / 60

Lect20 - 1 Lectures 20 Amino Acid Biosynthesis 2 Important...

This preview shows document pages 1 - 11. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online