7-Notes-NucleicAcidsTechniques(1)

7-Notes-NucleicAcidsTechniques(1) - Nucleic Acids 1 Sunday,...

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Nucleic Acids 1 Sunday, October 30, 2011
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Exploring genes and genomes (Chap. 5) DNA manipulation techniques • Technical tools • Recombinant DNA technology • Genomes • Eukaryotic genes 2 Sunday, October 30, 2011
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Exploring genes and genomes (Chap. 5) DNA manipulation techniques • Technical tools • Recombinant DNA technology • Genomes • Eukaryotic genes 3 Sunday, October 30, 2011
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What is recombinant DNA technology? 4 Sunday, October 30, 2011
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Bases of Recombinant DNA technology • Nucleic acid enzymology • SpeciFcity by base pairing • DNA sequencing techniques 5 Sunday, October 30, 2011
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Recombinant DNA technology • What are the tools used? • How do you clone a gene? 6 Sunday, October 30, 2011
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Recombinant DNA technology • What are the tools used? • How do you clone a gene? 7 Sunday, October 30, 2011
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DNA manipulation techniques • Restriction endonucleases • Blotting • DNA sequencing • DNA synthesis • PCR • Bioinformatics (Chapter 6) 8 Sunday, October 30, 2011
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DNA manipulation techniques • Restriction endonucleases • Blotting • DNA sequencing • DNA synthesis • PCR 9 Sunday, October 30, 2011
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What is a restriction endonuclease? 10 Sunday, October 30, 2011
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Sac II from S treptomyces ac hromogenes 11 Sunday, October 30, 2011
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Same DNA cut with different enzyme produces a unique restriction pattern. 12 Sunday, October 30, 2011
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13 Sunday, October 30, 2011
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14 Sunday, October 30, 2011
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DNA manipulation techniques • Restriction endonucleases • Blotting • DNA sequencing • DNA synthesis • PCR 15 Sunday, October 30, 2011
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7-Notes-NucleicAcidsTechniques(1) - Nucleic Acids 1 Sunday,...

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