GE - Gel Electrophoresis (SDS PAGE) Thomas G. Chasteen...

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Unformatted text preview: Gel Electrophoresis (SDS PAGE) Thomas G. Chasteen Department of Chemistry Sam Houston State University See http://www.shsu.edu/~chemistry/primers/pdf/GE.pdf for a PDF version of this document. Components The components of an SDS PAGE gel electrophoresis system are the following: A Slab holder for vertical or horizontal gels (thin, flat sheets of many individual lanes) Polyacrylamide or agarose gels (cm x cm x mm); these are poured for each analysis Gel is amended with SDS to dissociate & charge proteins. High voltage power supply (0.1-6 kV) A detection technique (dye staining, fluorescence, or autoradiography to image separated bands) Gel preparation Prepared samples (w/SDSsodium dodecyl sulfate) are pipetted into each of the wells of the gel/slab holder. Electrodes are already plumbed into the gel holder above and below the gel and they contact the gel via a liquid buffer (not shown). The connectors are electrically isolated from each other but contact opposite edges of thebuffer (not shown)....
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GE - Gel Electrophoresis (SDS PAGE) Thomas G. Chasteen...

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