lec10 - Biochemistry I Fall Term, 2004 September 22, 2004...

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1 Biochemistry I Fall Term, 2004 September 22, 2004 Lecture 10: Biochemical Energetics 2 Assigned reading in Campbell: Chapter 12.6-12.10. (Omit section 12.11) Key Terms: Liposomes Metabolism Anabolism Catabolism Oxidation-reduction reactions Coupling Links : ( I ) Review Quiz on Lecture 10 concepts ( O ) Protein Folding-Unfolding Transition: a Shockwave movie of an " αα structure" ( O ) Some Amino Acid Properties 12.6 Hydrophobic Interactions: A Case Study in Thermodynamics The descriptions in Campbell are a good introduction to the concepts involved in describing the formation of liposomes and the folding of proteins into their native conformation. The following sections add to those concepts some experimental results that use for their interpretation the relationships between G, H, S, and the equilibrium constant. A. Measurement of protein folding/unfolding equilibria For the reversible protein unfolding reaction, N <=> U with K eq = [U]/[N] the observed "transition curve" between native (N) and unfolded (U) states is a steep function of temperature, reflecting the cooperativity of the stabilizing forces. The examples below show the unfolding transitions of Protein G plotted as the fraction unfolded vs. the temperature. Curves for the wild type and two different mutant proteins are shown.
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2 Both the enthalpy ( H) and entropy ( S) of unfolding are available from these data. To obtain
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This note was uploaded on 03/04/2012 for the course BIOCHEM 451 taught by Professor Clavijo during the Spring '06 term at Carnegie Mellon.

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lec10 - Biochemistry I Fall Term, 2004 September 22, 2004...

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