MacVectorGettingStarted

MacVectorGettingStarted - Getting Started Copyright...

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Getting Started 2 MacVector Getting Started Copyright statement Copyright © 2001 Accelrys , a subsidiary of Pharmacopeia Inc. All rights reserved. This document contains proprietary information of Accelrys and its licensors. It is their exclusive property. It may not be reproduced or transmitted, in whole or in part, without written agreement from Accelrys . The software described in this document is furnished under a license agreement, a copy of which is packaged with the software. The software may not be used or copied except as provided in the license agreement. Accelrys reserves the right to make changes, without notice, both to this publication and to the product it describes. Information concerning products not manufactured or distributed by Accelrys is provided without warranty or representation of any kind, and Accelrys will not be liable for any damages. This version of Getting Started was published in August, 2001.
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Contents MacVector Getting Started 3 Contents Copyright statement 2 Contents 3 How to use Getting Started 4 Installing MacVector 4 Using this booklet 4 Outline of the experiment 5 After you finish this booklet 6 Starting MacVector 7 Construction of the human beta-globin sub-clone in pUC19 8 Importing sequence files 8 Working with features 10 Searching for restriction sites 12 Inserting the beta-globin gene into pUC19 19 Identifying restriction enzymes for Southern Blot analysis 23 Searching for restriction enzymes 23 Generating fragment predictions 25 Aligning sequences 28
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4 MacVector Getting Started How to use Getting Started This booklet contains step-by-step tutorials that are designed to introduce new users to the MacVector Sequence Analysis Software. Installing MacVector Refer to the Installation Notes for a step-by-step description of how to install MacVector. In this booklet it is assumed that MacVector is already installed. Using this booklet We recommend that you work through the tutorials in this booklet sequentially, using the MacVector program. The tutorials are based around a simple experiment and cover the following MacVector operations: working with sequence features viewing and editing sequence data searching for restriction sites aligning DNA sequences. You can find the example files used in this booklet in the MacVector/Tutorial Files folder. We recommend that you make a copy of the files contained in the MacVector/Tutorial Files folder, and put the copies in a convenient location for your use. Because you modify some of the tutorial files, this allows you and your colleagues to use the tutorials more than once, without the inconvenience of retrieving the tutorial files from the installation disk. ± NOTE
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This note was uploaded on 03/04/2012 for the course BIO 510 taught by Professor Miller during the Fall '06 term at Carnegie Mellon.

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MacVectorGettingStarted - Getting Started Copyright...

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