Spring 2004 Problem A3 Robert F. Murphy Page 1 of 1 Problem A3 BLAST Due: February 17, 2004 In this assignment, you are given a sequence of a mouse cDNA. Your goal is perform initial sequence analysis and comparison to find similar genes and proteins (see the MacVector manual for instruction son performing the various analyses). Hand in requested printouts and written answers to the questions. Include justification for your answers (i.e., show basis of calculations). Label your answers clearly. Questions (Total of 40 points) 1. Download the sequence file ProbA3.txt from the homeworks web page. Do a BLAST Search (use blastn) either from within MacVector or using the BLAST web page ( http://www.ncbi.nlm.nih.gov/BLAST/ ) to related nucleotide sequences. a) Print and submit the first two pages of the BLAST results. b) Is the cDNA sequence present in the Genbank database? If so, what is the name of the protein encoded by it? 2.
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This note was uploaded on 03/04/2012 for the course BIO 510 taught by Professor Miller during the Fall '06 term at Carnegie Mellon.