Computational Biology 03-310/510/710, Spring 2004R. F. MurphyPage 1 of 1Problem D1Finding objects in imagesDue: April 30, 2004 (accepted without penalty until May 11)You should use NIH Imageor ImageJpublic domain image analysis packages, and/or Matlab, for thisassignment. You will also need to download file(s) from the Homework Problems web page.Extra Credit Questions (10 points each)1.Open file giantin2.tif. This is an image from a fluorescence microscope where light regions (highpixel values) indicate the presence of fluorescence and the background is dark (low pixel values). Theimage is of cells that have been stained for the Golgi protein giantin. Invert the image so that thebackground is white and the regions with fluorescent dye are black. Threshold the image so that onlythe bright major objects are visible. Now use the Analyze Particles option to identify individual objectsin the image that have a minimum size of 2 pixels and a maximum size of 10,000 pixels (check the boxfor Label Objects). You should obtain 7 objects if your settings are correct.
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