Lecture 2 - BIO344: Molecular Biology Instructor: Scott W....

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TECHNIQUES AND TECHNOLOGIES BIO344: Molecular Biology Instructor: Scott W. Stevens, Ph.D.
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The basis for molecular biology One must be able to Isolate (purify)… Visualize… Manipulate… Assay… …molecules of interest
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How do we isolate biomolecules? DNA isolation Isolation requires cell lysis, protein denaturation/ digestion and alcohol precipitation. Cleaner DNA is achieved by CsCl purification / glass or silica binding
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How do we isolate biomolecules? DNA isolation Isolation requires cell lysis, protein denaturation/ digestion and alcohol precipitation. Cleaner DNA is achieved by CsCl purification / glass or silica binding
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How do we isolate biomolecules? DNA isolation Isolation requires cell lysis, protein denaturation/ digestion and alcohol precipitation. Cleaner DNA is achieved by CsCl purification / glass or silica binding
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How do we isolate biomolecules? DNA isolation Isolation requires cell lysis, protein denaturation/ digestion and alcohol precipitation. Cleaner DNA is achieved by CsCl purification / glass or silica binding
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How do we isolate biomolecules? RNA isolation Isolation requires cell lysis under highly unpleasant conditions, extraction or digestion of unwanted biomolecules, alcohol precipitation or silica binding.
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How do we isolate biomolecules? Protein isolation Isolation requires cell lysis under very gentle conditions, generally. Insoluble material and cell membranes are removed by centrifugation. Purification of proteins usually requires a series of different steps to isolate a pure sample.
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How do we isolate biomolecules? Protein isolation Purification techniques include: Precipitation: salt or organic chemicals can precipitate proteins in a general sense. Differential precipitation is a good first step for the purification of many proteins.
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Protein isolation
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This note was uploaded on 03/08/2012 for the course BIO 344 taught by Professor Herrin during the Spring '08 term at University of Texas at Austin.

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Lecture 2 - BIO344: Molecular Biology Instructor: Scott W....

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