Hist_Skin - Bio 348: General Histology and the Skin...

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Bio 348: General Histology and the Skin Introduction to the Microscope One very important component of the scientific method is the accurate and complete accounting of all observations. In fact, the field of biology is a relative late-comer to science because its development depended on the invention of instruments which could be used to extend the limitations of man's senses. Biology is an ever-expanding science (or you might say it's becoming more introspective) as more technologically advanced instruments are made available. At the cellular level many observations are performed with the aid of various types of microscopes. The advantages in scientific research provided by the microscope and its common usage require that you become confident in using and caring for it, as well as in your ability to accurately describe what you see. There are many different kinds of microscopes. Differences in the range of magnification are related to the intended purpose of the microscope. Stereoscopic light microscopes have a visual range of approximately 5cm to 0.005mm, 100 times more powerful than the naked eye. Stereoscopic microscopes are often used during dissections to study gross anatomy in three dimensions. Compound light microscopes have a visual range of approximately 1mm to 0.1micron (1 micron = 0.000001 meter), and are used to study very thin objects (like most cells) or sections of tissue in detail in two dimensions. There is yet a third kind of microcope which allows us to observe objects as small as a virus particle or even a protein molecule! This is the electron microscope which utilizes streams of electrons (rather than waves of much larger light particles called photons) to produce an image of the object. The microscope which you will use throughout this course is a delicate, expensive instrument. Please pay close attention to the directions for use given by your instructor, and take note of the following precautions: 1. When moved from one place to another, the microscope should always be held with two hands in an upright position. 2. Never touch the lens of the microscope with your hands. For cleaning the glass of ocular and objective lenses use only the special lens paper provided for this purpose. 3. Do not attempt to make any repairs yourself. Notify your instructor immediately if you have a problem with your scope. 4. Always locate the object to be studied with the low-power objective first, then turn to the high-power objective if more detailed study is desired. 5. Do not use the coarse focus adjustment when the high-power objective is in position. You may push the objective through the slide. 6. Never focus downward when looking through the ocular (eyepiece). Look from the side with the eyes at stage level. 7.
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Hist_Skin - Bio 348: General Histology and the Skin...

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