Slides_Phylogeny-2010S_1 - Carl Woese •  Wanted to know...

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Unformatted text preview: Carl Woese •  Wanted to know how all of life was related. •  Postulated to do this he would use a gene with the following properties: –  Found in all organisms –  Common function in all organisms –  Regions of variability and identity The gene for rRNA fit these criteria The Unusual Prokaryotes •  Methanogens –  eat CO2 and spew CH4 •  Thermophiles –  grew at very high temperatures Branch lines: end-points=today; start of line=start of life Norm Pace - Bioprospecting •  Applied PCR to amplify genes for 16S rRNA from environmental samples PCR ligate to plasmid transform E. coli Sequence plasmid DNA from each E. coli clone Diversity of the Ocean •  When the International Census of Marine Microbes (ICoMM) kicked off in 2003, microbiologists had identified 6,000 kinds of microbe and predicted that they might find as many as 600,000. But the latest analyses indicate that the oceans are home to at least 20 million types, including acantharians (pictured) — protists with skeletons made of strontium sulphate crystals. "The results just blow the wheels off all estimates of microbial diversity," says ICoMM leader Mitch Sogin of the Marine Biological Laboratory at Woods Hole, Massachusetts. See <> for more. ...
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