Reading_BacterialDevelopment_1 - Bacterial Development The...

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Unformatted text preview: Bacterial Development The following two readings from the textbook are examples of developmental processes that occur in bacteria that we will talk about in lecture. Textbook Reading Chapter 4, pages 96-98 “Endospores” The points to be remembered from this text: Know that some bacteria form endospores. Know that bacterial spores differ in form and function from vegetative cells. Know that sporulation proceeds through a series of discrete stages. Know that sporulation is a developmental process. Know that in sporulation a vegetative cell undergoes an asymmetric septation process. The larger cell engulfs the smaller cell. The smaller cell is called the forespore and the larger cell is called the mother cell. Textbook Reading Page 658 “The Cyanobacterial Heterocyst” The points to be remembered from this text: Know that cynobacteria form heterocysts. Know that heterocysts differ in form and function from be vegetative cells. Know that heterocysts fix dinitrogen and provide a reduced form of nitrogen to the vegetative cells and that the vegetative cells fix carbon dioxide and provide a reduced form of carbon to the heterocysts. To study cell specific gene expression or protein function (e.g. in the forespore versus the mother cell or the heterocyst versus the vegetative cell), fluorescent microscopy and a fluorescent reporter is used. For example, by creating a transcription fusion of a gene required for sporulation to the gene for the green fluorescent protein (GFP), one can determine whether that gene is expressed in the mother cell, the forespore or both. Textbook Reading Chapter 4, pages 81-82 “Fluorescence Microscope” The points to be remembered from this text: Know that fluorescence is the emission of light of a longer wavelength than that used to illuminate a small molecule or protein. Know that there are fluorescent dyes and proteins. Know that fluorescent microscopes are used to view fluorescent cells. Fluorsecent microscopes can be adjusted to emit a particular wavelength of light and detect a particular wavelength of light. 1 ...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.

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