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To study cell specific gene expression or protein

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Unformatted text preview: ocysts. To study cell specific gene expression or protein function (e.g. in the forespore versus the mother cell or the heterocyst versus the vegetative cell), fluorescent microscopy and a fluorescent reporter is used. For example, by creating a transcription fusion of a gene required for sporulation to the gene for the green fluorescent protein (GFP), one can determine whether that gene is expressed in the mother cell, the forespore or both. Textbook Reading Chapter 4, pages 81-82 “Fluorescence Microscope” The points to be remembered from this text: Know that fluorescence is the emission of light of a longer wavelength than that used to illuminate a small molecule or protein. Know that there are fluorescent dyes and proteins. Know that fluorescent microscopes are used to view fluorescent cells. Fluorsecent microscopes can be adjusted to emit a particular wavelength of light and detect a particular wavelength of light. 1...
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