Unformatted text preview: ocysts.
To study cell specific gene expression or protein function (e.g. in the forespore versus the mother
cell or the heterocyst versus the vegetative cell), fluorescent microscopy and a fluorescent
reporter is used. For example, by creating a transcription fusion of a gene required for
sporulation to the gene for the green fluorescent protein (GFP), one can determine whether that
gene is expressed in the mother cell, the forespore or both.
Textbook Reading Chapter 4, pages 81-82 “Fluorescence Microscope” The points to be remembered from this text:
Know that fluorescence is the emission of light of a longer wavelength than that used to
illuminate a small molecule or protein.
Know that there are fluorescent dyes and proteins.
Know that fluorescent microscopes are used to view fluorescent cells. Fluorsecent
microscopes can be adjusted to emit a particular wavelength of light and detect a
particular wavelength of light. 1...
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- Summer '10
- DNA, sporulation, vegetative cells