QuestionsBactDev

Questionsbactdev

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Unformatted text preview: Questions for Lecture on Bacterial Development 1. The spoIIQ gene is required for sporulation by Bacillus subtilis (i.e. B. subtilis mutants that lack the spoIIQ gene do not form spores). You hypothesize that spoIIQ is only expressed in cells that are undergoing sporulation and not in vegetative cells. Which of the following techniques could you use to detect spoIIQ expression to test this hypothesis: a) Northern blot with a probe specific to spoIIQ b) measure ß-galactosidase activity in cells that have a spoIIQ-lacZ transcriptional fusion c) monitor fluorescence in cells that have a spoIIQ-gfp (i.e. green fluorescent protein) transcriptional fusion d) none of the above e) all of the above 2. (continuing from question 1) You find that spoIIQ is not expressed in vegetative cells but in sporulating cells. Now, you want to know whether spoIIQ is expressed in the mother cell or the forespore. Which of the following techniques could you use to detect spoIIQ expression to address this question: a) Northern blot with a probe specific to spoIIQ b) measure ß-galactosidase activity in cells that have a spoIIQ-lacZ transcriptional fusion c) monitor fluorescence in cells that have a spoIIQ-gfp...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.

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