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Questions–TwoComponentSystems

Questions–TwoComponentSystems - Questions...

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Questions to Two Component Regulatory Systems and Environmental Sensing 1. The addition of glycine to the growth medium of B. lictus results in increased transcription of the glyC gene. A mutant strain of B. lictus that has a loss-of-function mutation in the glyR gene expresses higher levels of glyC than the wild-type strain in the absence of glycine. There is no difference in glyC expression in the presence of glycine. This data is represented in the following graph: A) The protein encoded by glyR is involved in transcriptional [activation/ repression ] of glyC . B) What experiment would you perform with purified GlyR protein to determine whether it could directly control transcription of the glyC gene? Please indicate the key components that would go into this experiment. (Note: you do not need to indicate what potential results would be, but the experimental set up should include a control.) 2. If a two-component regulatory system is activated by a small molecule, that small molecule most likely binds to the [kinase/response regulator] component. 3. In response to an activating environmental signal, what component of the two-component regulatory system becomes phosphorylated? a) kinase b) response regulator c) both the kinase and response regulator 4. Transcription of the ureA gene of V. crestus is induced by the presence of urea. In mutant strains of V. crestus
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