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Unformatted text preview: Questions and Answers for Viruses Part 1
1. Which of the following classes of viruses are able to use the host polymerase only to replicate
the virus genome (circle all that apply)?
c) (+) ssRNA
d) (+) ssRNA (retrovirus)
e) (-) ssRNA
2. The genome for a (+) ssRNA eukaryotic virus is substrate for both the ribosomes and the
RNA-dependent, RNA polymerase. How can a virus prevent competition between these two
enzymes for the genome? 3. You have identified a dsDNA virus that has terminal repeats in its DNA sequence. You
suspect that this virus replicates its genome using a “join and cut” mechanism. What is one
unique enzyme that you expect this viral genome to encode for genome replication? 4. You have identified a dsDNA virus that replicates via rolling circle replication. The diagram
below shows a copy of the viral genome that was cyclized and then nicked. The 5’ and 3’ ends
of the DNA are shown. Indicate on which strand lagging strand DNA synthesis will occur and
on which strand leading strand DNA synthesis will occur. 5. You have identified a virus that has a ssRNA genome. You want to know whether this viral
genome is an ssRNA(+) genome or a ssRNA(-) genome. You have a preparation of pure viral
particles (i.e. no cellular material) that you have broken open. What would you look for in these
viral particles that would lend support for the genome being (+) or (-) stranded? 6. An RNase (RNA-degrading enzyme) is critical for conversion of a ssRNA (+) retroviral
genome to its dsDNA form. How does this RNase avoid complete degradation of the ssRNA
genome prior to the production of the dsDNA form? ...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.
- Summer '10