Questions_VirusesPart1

Questions_VirusesPart1 - Questions and Answers for Viruses...

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Unformatted text preview: Questions and Answers for Viruses Part 1 1. Which of the following classes of viruses are able to use the host polymerase only to replicate the virus genome (circle all that apply)? a) dsDNA b) ssDNA c) (+) ssRNA d) (+) ssRNA (retrovirus) e) (-) ssRNA 2. The genome for a (+) ssRNA eukaryotic virus is substrate for both the ribosomes and the RNA-dependent, RNA polymerase. How can a virus prevent competition between these two enzymes for the genome? 3. You have identified a dsDNA virus that has terminal repeats in its DNA sequence. You suspect that this virus replicates its genome using a “join and cut” mechanism. What is one unique enzyme that you expect this viral genome to encode for genome replication? 4. You have identified a dsDNA virus that replicates via rolling circle replication. The diagram below shows a copy of the viral genome that was cyclized and then nicked. The 5’ and 3’ ends of the DNA are shown. Indicate on which strand lagging strand DNA synthesis will occur and on which strand leading strand DNA synthesis will occur. 5. You have identified a virus that has a ssRNA genome. You want to know whether this viral genome is an ssRNA(+) genome or a ssRNA(-) genome. You have a preparation of pure viral particles (i.e. no cellular material) that you have broken open. What would you look for in these viral particles that would lend support for the genome being (+) or (-) stranded? 6. An RNase (RNA-degrading enzyme) is critical for conversion of a ssRNA (+) retroviral genome to its dsDNA form. How does this RNase avoid complete degradation of the ssRNA genome prior to the production of the dsDNA form? ...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.

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