Questions_Transport

Questions_Transport - Transporters 1. (Choose one.)...

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Unformatted text preview: Transporters 1. (Choose one.) Transport of HPO4 along with a proton (H+) is an example of a. antiport. b. symport. c. uniport. d. facilitated diffusion. e. group translocation. 2. (Choose one.) The energy required for transport via ABC transporters is derived from a. a proton gradient. b. an electrical charge separation across the membrane. c. ATP hydrolysis. d. a symport mechanism. e. the high phosphate bond of PEP. 3. Many bacteria produce alcohols from sugar in the process of energy generation. These alcohols are toxic to cells. Alcohols can passively diffuse across a membrane, but several bacterial species have an antiporter to export alcohols. What advantage would having an alcohol-specific antiporter provide for these bacteria versus those bacteria that simply relying on passive diffusion of alcohols across the membranes? 4. You have two different bacterial species that both utilize glucose as a sole carbon and energy source. Species A uses a PTS system transporter for glucose; whereas species B uses an ABC transporter for glucose. You mix a small, but equal numbers of cells together of species A and species B and grow them together in a defined medium with glucose as the sole carbon and energy source. You incubate this mixed culture to allow growth of the cells, at the end of growth do you expect: (choose one) a) equal numbers of species A and species B cells b) greater number of species A cells c) greater number of species B cells 5. You have two different bacterial species, one of which, species A, uses a symporter to transport glucose and the second of which, species B, uses an ABC transporter for glucose. You mix equal amounts of the species A and species B cells in a defined minimal medium containing 10 µM glucose (i.e. a low concentrations) as the sole carbon source. After incubation for several hours, would you expect to find: (Choose one) a) equal numbers of species A and species B cells b) greater number of species A cells c) greater number of species B cells 6. You have found that the soil bacterium, P. fluorescens, can use the pesticide KillX as a source of Nitrogen for growth. You hypothesize that KillX is actively transported into the cell. Outline an experiment to test your hypothesis. Be sure to include a control in your experiment. (7 points) ...
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