Questions_Cult&Ident - Questions for the Cultivate...

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Unformatted text preview: Questions for the Cultivate and Identify Lecture 1. Enrichment culture procedures for many microorganisms require initial growth in liquid media, followed by growth on solid media. The solid media in this case is used to a. provide proper growth conditions. b. get rid of competing microorganisms. c. isolate strict aerobes. d. enrich for specific nutrients. e. isolate single colonies of the microbes. 2. An advantage of using liquid enrichment is that a. fast-growing organisms are enriched. b. slow-growing organisms are enriched. c. anaerobic organisms are enriched. d. aerobic organisms are enriched. e. a mixed population is enriched. 3. You want to isolate a microbe that can break down the trichloroethylene (TCE). You design a growth medium in which TCE is the sole source of carbon present. Briefly, indicate the method you would use to isolate, in pure culture, the most common TCE degrading microbe in your environmental sample. 4. You want to isolate a bacterium that uses cellulose as a carbon and energy source. You have a soil sample taken from a field with decaying plants. You hypothesize that your desired bacterium will be relatively rare in this sample. You design a defined growth medium whose only added source of carbon is cellulose. a) Indicate whether you would use a liquid or solid form of the growth medium to enrich for cellulose-degrading bacteria and Why? b) You determine that all the bacteria isolated are Geobacillus stearothermophilus. You want to know whether these are all the same strain of G. stearothermophilus or whether they are different. Outline a procedure to determine the relatedness of the different G. stearothermophilus isolates. ...
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This note was uploaded on 03/06/2012 for the course MIMG 100 taught by Professor Lazazzera during the Summer '10 term at UCLA.

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